Research activities

Development of methods for determination of oxidative stress dependent diseases, mass spectrometry


Since 2011: ICT Prague, Drugs and Biomaterials PhD program
2009 - 2011: ICT Prague, Drug Production master degree program
Thesis title: Method development for monitoring of protein and nucleic acid damage as a consequence of oxidative stress
Since 2007: Member of the research team at the Department of Organic Technology, ICT Prague
2006 - 2009: ICT Prague, Drug Production and Synthesis bachelor degree program
Thesis title: Determination of DNA, RNA and protein deterioration biomarkers in cells as a consequence of oxidative stress


7/2011 - 12/2011: Internship at Fachhochschule Nordwestschweiz in Basel (Switzerland)
Topic: Improvement of the dissolution properties of poorly water soluble substances using the spray drying technique


2010: 1st place at Student Scientific Conference – ICT Prague, Czech Republic
3rd place in the „Shimadzu Prize“ – Pardubice, Czech Republic
2009: 3rd place at Student Scientific Conference – ICT Prague, Czech Republic


2. Syslová, K.; Böhmová, A.; Demirbağ, E.; Šimková, K.; Kuzma, M.; Pelclová, D.; Sedlák, V.; Čáp, P.; Martásek, P.; Kačer, P. Immunomagnetic molecular probe with UHPLC-MS/MS: A promising way for reliable bronchial asthma diagnostics based on quantification of cysteinyl leukotrienes. J. Pharm. Biomed. Anal. 2013, 81–82, 108–117.
DOI: 10.1016/j.jpba.2013.03.026
1. Syslová, K.; Kačer, P.; Kuzma, M.; Pankrácová, A.; Fenclová, Z.; Vlčková, S.; Lebedová, J.; Pelclová, D. LC-ESI-MS/MS method for oxidative stress multimarker screening in the exhaled breath condensate of asbestosis/silicosis patients. J. Breath Res. 2010, 4 (1), 017104/1-017104/8.
DOI: 10.1088/1752-7155/4/1/017104


2012: 30th Informal Meeting on Mass Spectrometry (poster) – Olomouc, Czech Republic
2010: 62nd Congress of Czech and Slovak Chemical Companies – Pardubice, Czech Republic
Student scientific conference (talk) – ICT Prague, Czech Republic
2009: Student scientific conference (talk) – ICT Prague, Czech Republic

Current research project

This research project concerns multimarker screening of exhaled breath condensate (EBC) for diagnostics of lung diseases. Aim of this project is to develop methods for determination of a maximum number of known biomarkers in EBC with a minimum number of analysis. Simultaneously, new and at present unknown substances in EBC which could be used as biomarkers of lung diseases will be determined.