Research activities

Magnetic nanoparticles, lateral flow immunoassay, mass spectrometry

CV

Since 2011: ICT Prague, Organic Technology PhD program
2009 - 2011: ICT Prague, Drug Production master degree program
Thesis title: Preparation of immunomagnetic molecular nano-probe for biomarker separation from body fluids
Since 2008: Member of the research team at the Department of Organic Technology, ICT Prague
2006 - 2009: ICT Prague, Drug Production and Synthesis bachelor degree program
Thesis title: Preparation of immunomagnetic nanoparticles for biomarker separation from exhaled breath condensate

Internships

1/2012 - 6/2012: Internship at Fachhochschule Nordwestschweiz in Basel (Switzerland)
Topic: Spray drying of milk-thistle extract

Prizes

2010: Scholarship of Preciosa Foundation, academic year 2010/2011
2009: 1st place at Student Scientific Conference – ICT Prague, Czech Republic

Publications

1. Syslová, K.; Böhmová, A.; Demirbağ, E.; Šimková, K.; Kuzma, M.; Pelclová, D.; Sedlák, V.; Čáp, P.; Martásek, P.; Kačer, P. Immunomagnetic molecular probe with UHPLC-MS/MS: A promising way for reliable bronchial asthma diagnostics based on quantification of cysteinyl leukotrienes. J. Pharm. Biomed. Anal. 2013, 81–82, 108–117.
DOI: 10.1016/j.jpba.2013.03.026

Conferences

2010 Shimadzu Awards (talk) – Pardubice, Czech Republic
Student scientific conference (talk) – ICT Prague, Czech Republic
2009: Student scientific conference (talk) – ICT Prague, Czech Republic

Current research project

In my doctoral work I focus on the development of medicinal diagnostic tools, particularly on the preparation of immunomagnetic nanoparticles and diagnostic strips. Both methods represent a quick way of determination of serious civilization diseases (e.g. asthma, carcinomas) in biological matrices (blood plasma, exhaled breath condensate, cerebrospinal fluid, urine). For that purpose, a monitoring of substances produced during ongoing pathological processes, so called biomarkers, is used. The preparation of magnetic nanoparticles consists of a) synthesis of the magnetic core by alkaline co-precipitation of Fe2+/Fe3+ ions, b) stabilization of nanoparticles using polymers (usually chitosan) and c) characterization by various physico-chemical methods (TEM, AFM, XRD, DLS, IR, TGA). In the next step, molecular structures like monoclonal antibodies, are anchored onto the nanoparticles of uniform parameters. These structures ensure the specificity towards biomarkers. The prepared particles are used for the immunomagnetic separation of biomarkers from body fluids and the acquired sample is subsequently analyzed by HPLC-ESI-MS/MS.

Further part of my work deals with the development of a strip for diagnostics, which will allow approximate determination of diseases within several minutes, and which is based on a lateral flow immunoanalysis. The diagnostic strip consists of primary antibodies against monitored biomarkers, which are immobilized on a porous polymeric carrier (e.g. nitrocellulose), secondary antibodies, a substrate anchored on polymeric carrier and antibodies labeled with gold nanoparticles. The concentration of monitored biomarkers is discovered owing to the color change of the strip, which is caused by the creation of an antigen-antibody linkage. This method should be used especially in prophylaxis of diseases as it allows determining of an ongoing pathological process in a body in an asymptomatic period. Home testing by people inheritably predisposed to certain illnesses or during preventive check-ups is expected.