Research activities

Research and development of novel platinum cytostatics

CV

Since 2011: ICT Prague, Organic Technology PhD program
2009 - 2011: ICT Prague, Drug Production master degree program
Thesis title: Preparation of (R-amidine)PtClx compounds as potencial platinum cytostatics
Since 2010: Member of the research team at the Department of Organic Technology, ICT Prague
2006 - 2009: ICT Prague, Drug Production and Synthesis bachelor degree program
Thesis title: Preparation of calix[4]arenes bearing amidinium groups

Internships

8/2010: Internship in the QC laboratories of PRO.MED.CS

Publications

1. Budka, J.; Eigner, V.; Holakovský, R.; Kovaříček, P.; Loužilová, T. 25,26,27,28-Tetrapropoxycalix[4]arene-5,17-dicarbonitrile. Acta Crystallogr. 2010, E66, o419–o420.
DOI: 10.1107/S1600536810002242

Conferences

2010: Student scientific conference (talk) – ICT Prague, Czech Republic

Current research project

The work is focused on the praparation of precursors of platinum compounds used for the production of cytostatics of the 4th generation, also called analogues of picoplatin. Pyridines with the amidine group in positions α, β and γ were used as the starting compounds. Platinum complexes of these amidines have not been reported yet, and because of their structural similarity with picoplatin, they represent an interesting group of potential cytostatic drugs. The primary field of our interest was to understand the mechanistic causalities and to find suitable conditions for the preparation of these platinum complexes. We managed to synthesize the platinum complexes using the reactants with the amidine group bound in position α. These Pt-amidine compounds were tested in the XTT test in two human tumor cell lines, originating from breast cancer (MCF-7) and colon carcinoma (CaCo-2), in comparison with the picoplatin, and we obtained a very promising efficacy. The Pt-β-amidine and Pt-γ-amidine complexes have not been prepared yet. The procedure that was used to prepare the α-complexes is not suitable for the synthesis of complexes with the use of the parent compounds with the amidine group bound in positions β and γ (already experimentally verified). Therefore, it will be necessary to perform a new study on the impact of reaction conditions upon the formation of these complexes.