Ph.D. Studies

Development of a method for routine determination of psychotropic substances in the organism

Supervisor: Assoc. Prof. Dr. Petr Kačer

The work is oriented towards the development of rapid diagnostic kits for the detection of the presence/absence of psychotropic substances present in body fluids (urine, saliva). In the first step of the work it will be necessary to find appropriate metabolites of psychotropic substances in body fluids, which will be suitable for the semi-quantitative determination as well as for the visualization-detection response specific for a selected substance/metabolite. For the given metabolites, a diagnostic kit that will operate on the basis of antigen-antibody reaction with a colorimetric indication of antigen-antibody complexes will be created.


Development of a diagnostic strip for medicinal diagnostics

Supervisor: Assoc. Prof. Dr. Petr Kačer

The work is oriented to the preparation of a diagnostic strip for approximate determination of serious civilization diseases (e.g. asthma, various types of cancer) from body fluids. The diagnostic strip should consist of immobilized antibodies against specific substances characteristic for a particular disease (so-called "biomarkers") into the porous polymer carriers. The determination of concentrations of biomarkers should be based on the colour change of the strip as a result of the antigen-antibody bond being formed.


Development of a method for the diagnosis of Alzheimer's disease

Supervisor: Assoc. Prof. Dr. Petr Kačer

The work is oriented towards the development of a method based on mass spectrometry for the diagnosis and monitoring of progression of Alzheimer's disease. The work will focus on the development multimarker screening for the identification and quantification of a number of important biomarkers of Alzheimer's disease (markers of oxidative stress, neuroprostanes, tau-proteins, etc.). The principle of the developed methods should be a combination of suitable separation techniques (immunoextraction, solid phase extraction (SPE)) with a highly selective and sensitive detection by mass spectrometry.

Preparation of MIP for the separation of neurotransmitters from the brain

Supervisor: Assoc. Prof. Dr. Petr Kačer

The work is oriented towards the development of MIP (Molecule Imprinting Polymer) as a rapid and highly specific tool for the separation of neurotransmitters (dopamine, serotonin) and their metabolites from complex matrices (brain tissue and microdialyzate) prior to analysis based on mass spectrometry. The developed tool should serve for the separation of those substances and should be used in experimental studies to monitor brain function.

Development of magnetic nanoparticles for medicinal diagnostics

Supervisor: Assoc. Prof. Dr. Petr Kačer

The work is oriented to the preparation and characterization of magnetic nanoparticles functionalized with specific molecular structures that enable a highly selective separation of target structures (molecules produced during pathological processes in the body, called markers) from complex biological samples such as blood plasma, breath condensate, cerebrospinal liquor, urine, etc. The work is focused on both the process of preparing uniform magnetic nanoparticles and their characterization, and their testing in the diagnostic process or comparing them with current methods used in clinical practice.

Development of catalytic systems for stereoselective reduction of the C=N bond

Supervisor: Assoc. Prof. Dr. Petr Kačer

The work is focused in the area of preparation and characterization of new catalytic systems for the asymmetric hydrogenation of C=N double bond. The aim of this work is to develop new stereoselective homogeneous catalysts exhibiting appropriate parameters (stereoselectivity, activity, possibility of regeneration) for the application in pharmaceutical technology processes. The work will include both rational design of a catalytic system based on the method of similarity in molecular modeling and synthesis of selected structures and their subsequent kinetic testing with model substances containing C=N double bond in their structure. The work will be directed to a practical application, i.e. finding a catalytic system and its development into a form for industrial application in the technological process of production of modern muscle relaxants based on curare alkaloids.

Development of nanoparticles for targeted drug delivery

Supervisor: Assoc. Prof. Dr. Petr Kačer

The work is oriented to the area of preparation and characterization of functionalized gold nanoparticles for highly specific active transport of cytostatic drugs. The work will focus on both the preparation of gold nanoparticles and on their coating with structures enabling the 'acceptation' by the immune system. Finally, a method for capturing other structures on functionalized particles will be developed and standardized. These structures will be responsible for specific interaction ("uptake") with target molecular structures localized on tumor cells, as well as cytostatic-active molecules (drugs).

Development of new boron compounds for boronic capture therapy

Supervisor: Assoc. Prof. Dr. Petr Kačer

The aim of this project is to synthesize new potential substances rich in boron useful in boronic capture therapy (BNCT), which will show specificity for tumor cells, but also it will be the structure of a medium molecular weight, which will not be subject to significant accumulation in the liver. Work will focus on:
* Design and construction of suitable boron compounds applicable for BNCT therapy of tumors, especially of brain.
* Usage of appropriate structures to increase the selectivity of potential drugs for selected types of tumors.
* Designing the final dosage form for the administration of drugs.

Personalized medicine of lung diseases – a new direction in medicinal diagnostics

Supervisor: Assoc. Prof. Dr. Petr Kačer

The work is focused on the area of personalized medicine as a new direction in medical diagnosis. The personalized medicine uses genomic and molecular analysis of body fluids and tissues for the diagnosis of pathological processes and therapy monitoring. For its expansion into routine medical practice in the diagnosis of many diseases, it is necessary to identify specific substances characteristic for a particular disease – the so-called "biomarkers". Methods combining separation of biomarkers from complex biological matrices (urine, blood plasma, cerebrospinal fluid, exhaled breath condensate) with a highly sensitive and accurate method for mass spectrometry will be developed for the detection and quantification of these compounds.

Preparation of new platinum chemotherapeutics of the fourth generation

Supervisor: Assoc. Prof. Dr. Petr Kačer

The work is oriented to the preparation of new platinum complexes on a laboratory scale. At present, the research and development is oriented to so-called platinum chemotherapeutics of the fourth generation (e.g. picoplatin). Prior to experimental work, a detailed literature review focused on the use of Pt(II) complexes with cytostatic activity will be worked out. The review will also be processed on the new direction in the treatment of tumors – the use of Pt(IV) complexes. In the work, reaction conditions of the preparation of various complexes to prepare analogues picoplatin and oxaliplatin with platinum in oxidation state (IV) will be examined.